Stillingstittel:
PhD candidate/Research Fellow (Ref.nr. 3061928493)

Stillingsbeskrivelse:
Oslo University Hospital is a highly specialized hospital in charge of extensive regional and local hospital assignments. As Scandinavia’s largest hospital, we carry out more than 1.2 million patient treatments each year. The hospital has a nationwide responsibility for a number of national and multi-regional assignments, and has several national centers of competence. Oslo University Hospital is responsible for approximately 50 percent of all medical and health care research conducted at Norwegian hospitals, and is a significant role player within the education of a large variety of health care personnel.Center for Eye Research (CER), Dep. of Ophthalmology, HHA, Oslo University Hospital/ University of Oslo.There is a vacancy for a position as PhD candidate/Research Fellow for 3 years at the - HHA forskningsfløyen

The Department of Ophthalmology, Oslo University Hospital consists of a total staff of 220 persons including 60 physicians, ten operating theatres and a ward of 19 beds, a large ophthalmology department even in European terms. Its main research objective is to improve current treatment and to develop new therapeutic modalities for selected eye disorders. The focus is on: 1) clinical research with a large patient population, as well as 2) translational research - clinically relevant laboratory research at CER with an interdisciplinary staff possessing a wide range of expertise. The CER/The Norwegian Eye Bank is located at the Department of Ophthalmology. CER has wide experience in primary and stem cell isolation from the eye, cell culture procedures, tissue/organ culture for transplantation, immunohistochemistry, electron microscopy, studies at gene- and protein- level, RNA in situ hybridization, 4D Nucleofector transfection and MACS facilities. Epigenetic characterization by examining DNA methylation patterns and histone modifications are also performed. One of the advantages of CER is its close relation to the clinics, its research and ex vivo tissue engineering facilities being located near the operating theatres and the ward. As the first clinic in Scandinavia, the Department of Ophthalmology/CER are offering transplantation of autologous ex vivo expanded human corneal epithelial tissue to patients with Limbal Stem Cell Deficiency (LSCD). Much research is presently taken to standardize further the safety and viability of the transplanted cell therapy bioscaffolds, with progress made towards implementing new GMP technology. CER is a founding member of the Norwegian Center for Stem Cell Research (www.stemcellnorway.org ) and a member of the Oslo Regenerative Medicine Initiative. It has active collaboration with other laboratories both nationally and internationally. Webpage:http://www.med.uio.no/klinmed/forskning/grupper/senter-for-oyeforskning/

Job description

• Targeting epigenetic status of human limbal epithelial stem cells to improve ocular surface reconstruction.
• Main objective of the project: Increase the quality and number of stem cells in ex vivo expanded human limbal epithelial cells (HLECs) for future clinical transplantation. The outcome after transplantation of ocular surface epithelium is significantly related to the quality of the engineered tissue. The main goal of the project is to reveal molecular pathways that might increase the number of cells expressing markers associated with stemness in our HLECs expansion protocol. In addition, we will train a candidate in the wide range of primary and stem cell isolation, cultivation and characterization techniques in both, anterior and posterior, segments of the eye.

Qualifications

• Applicants must hold a degree equivalent to a master's degree or higher within a relevant area, such as cell- or molecular biology, (epi)-geneti
  
  Minstelønn: 0
  
  Maksimal lønn: 0
  
  Arbeidsgiver Navn: Oslo Universitetssykehus HF, Ullevål
  
  Arbeidsgiver adresse: 0450 OSLO
  
  Job kort beskrivelse: University and higher education teachers
  
  Land: Norge
  
  Region: Oslo
  
  Kilde til jobbtilbud: NAV, Offentlige arbeidsformidlinger, Norge
  
  Lønn valuta: NOK
  
  Lønn periode: Pr. år
  
  
  GJELDER